CRISPR-Cas: A Laboratory Manual by Jennifer Doudna
- CRISPR-Cas: A Laboratory Manual
- Jennifer Doudna
- Page: 450
- Format: pdf, ePub, mobi, fb2
- ISBN: 9781621821311
- Publisher: Cold Spring Harbor Laboratory Press
Download CRISPR-Cas: A Laboratory Manual
Download free kindle books for android CRISPR-Cas: A Laboratory Manual FB2 RTF 9781621821311
CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.
Richard Behringer on Twitter: "New lab manual on CRISPR/Cas
New lab manual on CRISPR/Cas protocols edited by Doudna & Mali @ CSHLPress due March 2016 http://bit.ly/1OZiyf4 pic.twitter.com/
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CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells
Cold Spring Harbor Lab Press Genomics
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
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